step,stream,direction,water_kg,substrate_kg,biomass_kg,product_kg,total_kg,evidence_class,student_task
fermentation,basal_medium,in,900,100,0,0,1000,given,Define the component basis
fermentation,inoculum,in,45,4,1,0,50,given,Add the second inlet to the ledger
fermentation,harvest_broth,out,930,20,60,25,1035,calculated,Reconcile the reaction conversion
fermentation,vent_and_evaporation,out,15,0,0,0,15,assumption,Test the effect of evaporation uncertainty
clarification,harvest_broth,in,930,20,60,25,1035,calculated,Carry the reconciled upstream stream forward
clarification,clarified_pool,out,900,18,3,24,945,calculated,Calculate product recovery and biomass removal
clarification,wet_solids,out,30,2,57,1,90,calculated,Check total and component closure
sterile_filtration,clarified_pool,in,900,18,3,24,945,calculated,Define the filtration feed basis
sterile_filtration,filtered_pool,out,890,16,0.5,23.5,930,calculated,Calculate product recovery and residual biomass
sterile_filtration,filter_hold_up_and_waste,out,10,2,2.5,0.5,15,assumption,Run a low and high hold-up scenario